Cyclic Citrullinated Peptide High Purity Spec Sheet
Executive Summary
Cyclic Citrullinated Peptide High Purity Spec Sheet provides researchers with a precisely characterized reagent for autoimmune assay development. This product is manufactured under strict cGMP guidelines, ensuring batch-to-batch consistency and a minimum purity of 98% verified by HPLC and mass spectrometry. Designed for diagnostic kit calibration and immunoassay validation, it eliminates common buyer concerns such as lot variability and cross-reactivity interference. Each lot undergoes rigorous quality control testing to confirm structural integrity and peptide content, addressing the pain point of unreliable raw materials. By offering a documented spec sheet with clear analytical data, this peptide supports reproducible results in clinical research settings. It is intended for laboratory use only and not for diagnostic or therapeutic procedures.
Target Keyword: cyclic citrullinated peptide
Core Molecular Specs & Product Technical Index
Cyclic citrullinated peptide is a synthetic cyclic peptide antigen that contains the modified amino acid citrulline. This structural feature is critical for its specific binding affinity to autoantibodies found in certain rheumatic conditions. Our product is manufactured to a minimum purity of 98%, verified by reversed-phase HPLC and confirmed by electrospray ionization mass spectrometry (ESI-MS). The peptide is supplied as a lyophilized powder with a net peptide content of ≥85%, ensuring accurate reconstitution for assay calibration. Solubility is optimized in deionized water or phosphate-buffered saline (PBS) at pH 7.4, with a recommended storage temperature of -20°C to maintain long-term stability. All batches comply with the national industry testing standard for synthetic peptide reagents, including residual solvent analysis and endotoxin testing.
- Purity: ≥98% by HPLC (UV detection at 214 nm)
- Molecular Weight: Confirmed by ESI-MS within ±0.5 Da of theoretical value
- Peptide Content: ≥85% (by amino acid analysis)
- Solubility: ≥10 mg/mL in water or PBS (pH 7.4)
- Storage: Lyophilized powder stable at -20°C for 24 months
According to a 2023 market analysis by Grand View Research, the global autoimmune diagnostic reagents market is projected to reach USD 8.2 billion by 2030, with cyclic citrullinated peptide-based assays representing a significant growth segment due to their high specificity in rheumatoid arthritis serology.
Manufacturing Flow & Strict Quality Control System
Our production process begins with rigorous raw material screening, where each amino acid derivative is tested for purity and chirality before synthesis. The peptide is assembled using solid-phase peptide synthesis (SPPS) on a controlled pore glass resin, followed by cyclization in solution to form the disulfide bridge that stabilizes the cyclic structure. Multi-stage purification is performed using preparative HPLC with a C18 column, achieving baseline separation of the target peptide from truncated sequences and deletion impurities. Each lot undergoes third-party testing for identity, purity, and bioactivity, with a certificate of analysis (COA) provided. Long-term stability studies under accelerated conditions (40°C/75% RH) confirm that the peptide retains >95% purity for 6 months, ensuring reliable performance in diagnostic kit manufacturing.
Main Certification Standards We Support
- ISO 9001:2015 Quality Management System certification
- cGMP compliance for raw material production
- USP <1058> analytical instrument qualification
- ICH Q1A(R2) stability testing guidelines
- ISO 17025 accredited laboratory testing
Main Commercial Application Scenarios
Cyclic citrullinated peptide is primarily used as a key reagent in the development and calibration of immunoassays for autoimmune disease research. In cosmetic formulation, it serves as a bioactive ingredient in anti-aging serums, where its cyclic structure enhances skin penetration and collagen synthesis stimulation. For laboratory research, it is employed as a positive control in ELISA and Western blot assays to validate antibody specificity. In compound raw material wholesale, it is supplied in bulk quantities to diagnostic kit manufacturers who require consistent lot-to-lot performance for commercial test kits.
- Diagnostic Kit Calibration: Used as a calibrator in anti-CCP ELISA kits for rheumatoid arthritis testing
- Cosmetic Formulation: Incorporated into peptide-based serums at 0.1-1.0% concentration for anti-aging benefits
- Research Reagent: Positive control for autoantibody detection in autoimmune disease studies
- Bulk Raw Material: Supplied in 1g to 100g quantities for kit manufacturing
Comparison: cyclic citrullinated peptide VS Ordinary Low-Grade Peptide Raw Materials
| Item | Our High-Purity Product | Common Alternatives | Advantage |
| Purity | ≥98% by HPLC | 85-92% by HPLC | Higher specificity reduces false positives |
| Batch Consistency | CV <5% across lots | CV 10-20% across lots | Reliable assay calibration |
| Endotoxin Level | <0.5 EU/mg | 1-5 EU/mg | Suitable for cell-based assays |
| Stability | 24 months at -20°C | 6-12 months at -20°C | Longer shelf life reduces waste |
| Documentation | Full COA with HPLC, MS, AA analysis | Basic COA with only purity | Complete traceability for audits |
Standard Buyer Selection Guide For Bulk Purchase
When purchasing cyclic citrullinated peptide in bulk, common pitfalls include accepting low-purity material that causes assay variability and relying on suppliers without third-party testing. Core screening criteria include verifying the supplier's ISO certification, requesting a sample for in-house validation, and confirming that the peptide content is specified on the COA. Supplier qualification should include a site audit or review of their quality management system, as well as checking for any history of product recalls or contamination issues.
Key Inspection Checklist Before Cooperation
- Request a certificate of analysis (COA) with HPLC chromatogram and mass spectrum
- Verify that the supplier provides batch-specific stability data
- Confirm that the peptide is manufactured under cGMP conditions
- Check for independent third-party testing results (e.g., amino acid analysis)
- Ensure that the supplier offers a sample for pre-purchase validation
Core Competitive Advantages Of Our cyclic citrullinated peptide
Our product offers superior purity of ≥98%, which directly translates to higher specificity in diagnostic assays and reduced background noise in research applications. The batch-to-batch consistency, with a coefficient of variation below 5%, ensures that your assay results remain reproducible over time. Cost performance is optimized through our efficient synthesis process, which reduces production waste and allows competitive pricing for bulk orders. After-sales technical support includes access to our quality control team for troubleshooting and custom formulation requests, providing a partnership that extends beyond a simple transaction.
Frequently Asked Questions
Q: What is the recommended storage condition for cyclic citrullinated peptide to maintain its stability?
A: The lyophilized powder should be stored at -20°C in a desiccated environment, protected from light. Under these conditions, the peptide remains stable for up to 24 months. Once reconstituted, it should be used within 24 hours if stored at 4°C, or aliquoted and frozen at -20°C for longer storage, avoiding repeated freeze-thaw cycles.
Q: How do you verify the cyclic structure of the peptide in your quality control process?
A: The cyclic structure is confirmed by mass spectrometry, where the observed molecular weight matches the theoretical value for the cyclic form, and by HPLC retention time comparison with a reference standard. Additionally, Ellman's test is performed to ensure the absence of free thiol groups, confirming that the disulfide bridge is intact.
Q: Can this peptide be used in cell culture experiments without causing cytotoxicity?
A: Yes, our peptide has an endotoxin level of less than 0.5 EU/mg, making it suitable for cell culture applications. However, we recommend performing a dose-response cytotoxicity assay in your specific cell line before routine use, as individual cell types may have different sensitivities to peptide concentrations.