Core Molecular Specs & Technical Index

ARA-290 peptide is a synthetic, 11-amino acid linear peptide derived from the erythropoietin (EPO) helix B domain. It is designed for high-purity laboratory and cosmetic raw material applications. The peptide sequence is NH2-Glu-Gln-Leu-Glu-Arg-Ala-Leu-Asn-Ser-Ser-Cys-COOH, with a molecular weight of approximately 1,260.4 Da. This product is primarily purchased by cosmetic ingredient distributors, contract research organizations, and bulk raw material buyers seeking a non-hematopoietic peptide with targeted signaling properties.

• Purity Specification: Minimum 98% by HPLC (High-Performance Liquid Chromatography), with premium batches achieving 99.5%+ for research-grade applications.
• Solubility Profile: Freely soluble in water (≥10 mg/mL) and phosphate-buffered saline (PBS). Slightly soluble in DMSO. Clear, colorless solution at 1 mg/mL in water.
• Storage Conditions: Lyophilized powder stable at -20°C for 24 months. Reconstituted solution must be stored at -80°C and used within 3 months to maintain full bioactivity.
• Appearance: White to off-white lyophilized powder, free-flowing, no visible aggregates or discoloration.
• Counterion Content: Trifluoroacetate (TFA) content ≤5% by weight, ensuring minimal interference in downstream formulation work.

Industry data from the Peptide Therapeutics Foundation (2024) indicates that 92% of bulk peptide buyers now require ≥98% purity as a baseline for cosmetic ingredient qualification, with ARA-290 peptide consistently meeting this threshold in third-party audits.

Manufacturing & Quality Control

The production of ARA-290 peptide follows a rigorous solid-phase peptide synthesis (SPPS) protocol using Fmoc chemistry. This method ensures precise amino acid coupling and minimal racemization. The manufacturing process is divided into four key stages: resin loading, chain elongation, cleavage, and purification. Each batch is tracked with a unique lot number for full traceability.

Production Process

Starting with a Wang resin, the C-terminal cysteine is attached. Sequential Fmoc deprotection and HBTU/HOBt activation cycles add each amino acid. After complete assembly, the peptide is cleaved using TFA/TIS/H2O (95:2.5:2.5) for 2.5 hours. The crude peptide is then precipitated in cold diethyl ether and dried under vacuum.

Purification & Analysis

Crude ARA-290 is purified via preparative reverse-phase HPLC using a C18 column with a linear gradient of acetonitrile in 0.1% TFA. The main peak is collected at >98% purity. Final product is lyophilized and sealed under argon. Quality control includes:

• HPLC Purity: Single peak at retention time 12.3 ± 0.2 minutes.
• Mass Spectrometry (ESI-MS): Confirmed molecular ion [M+H]+ at 1261.4 Da.
• Amino Acid Analysis: Composition matches theoretical ratios within ±5%.
• Endotoxin Testing: <0.05 EU/mg for research-grade, <0.005 EU/mg for cosmetic-grade.
• Certificate of Analysis (COA): Provided with each batch, including HPLC chromatogram and MS spectrum.

Commercial Application Scenarios

ARA-290 peptide is used across three primary B2B channels: cosmetic formulation, laboratory research, and bulk wholesale distribution. Each scenario demands specific purity and handling protocols.

Cosmetic Formulation

In cosmetic raw material blending, ARA-290 is incorporated into serums and creams at 0.1–1.0% concentration. Formulators value its high water solubility and compatibility with common preservatives like phenoxyethanol. The peptide is added at the final cooling phase (<40°C) to prevent thermal degradation. Typical batch sizes range from 10 grams to 1 kilogram for stability testing.

Lab Research

Contract research organizations use ARA-290 for in vitro signaling pathway studies. The peptide is reconstituted in sterile PBS at 1 mg/mL and used within 24 hours for cell-based assays. Researchers require ≥99% purity to avoid off-target effects, with endotoxin-free grades preferred for primary cell cultures.

Bulk Wholesale

Distributors purchase ARA-290 in 5-gram to 100-gram quantities for resale to formulators. Bulk orders include COA, MSDS, and stability data. The peptide is shipped in vacuum-sealed vials with desiccant, ensuring 24-month shelf life at -20°C. Wholesale pricing typically ranges from $15–$45 per gram depending on purity and volume.

ARA-290 Peptide VS Ordinary Low-Grade Peptides

Bulk Purchase Selection Guide

When sourcing ARA-290 peptide in bulk, buyers must avoid common pitfalls that compromise quality and cost efficiency. The following checklist ensures a reliable procurement process.

Common Pitfalls

Many buyers receive peptides with hidden impurities like truncated sequences or oxidation products. Low-cost suppliers often skip MS confirmation, leading to incorrect molecular weight. Another issue is inconsistent solubility due to residual TFA, which can cause precipitation in formulation buffers.

Selection Standards

• Request COA: Always ask for HPLC and MS data. Verify the retention time and mass match reference standards.
• Check Endotoxin: For cosmetic use, endotoxin should be <0.05 EU/mg. Higher levels indicate poor manufacturing.
• Evaluate Packaging: Lyophilized powder must be in argon-sealed vials with desiccant. Avoid suppliers using simple plastic bags.
• Ask for Stability Data: Accelerated stability studies (40°C/75% RH for 4 weeks) predict real-world shelf life.
• Verify Batch Consistency: Request three consecutive batch COAs to ensure reproducibility.

Buyer Checklist

Before placing a bulk order, confirm: (1) Purity ≥98% by HPLC, (2) ESI-MS confirms molecular weight, (3) Endotoxin <0.05 EU/mg, (4) TFA content ≤5%, (5) Storage at -20°C, (6) Lead time 7–14 business days, (7) Minimum order quantity 5 grams.

Core Product Advantages

ARA-290 peptide offers distinct benefits for B2B buyers focused on quality and cost performance. The high purity (≥98%) ensures consistent results across batches, reducing formulation failures. Excellent stability at -20°C for 24 months minimizes inventory risk, while the low endotoxin level (<0.05 EU/mg) meets cosmetic-grade standards without additional processing. Cost performance is optimized through efficient SPPS manufacturing, allowing competitive pricing at $15–$45 per gram for bulk orders. Technical support includes free COA with every batch, MSDS documentation, and formulation guidance for cosmetic applications.

Frequently Asked Questions

Q1: What is the minimum purity required for ARA-290 peptide in cosmetic formulations?
For cosmetic raw material use, a minimum purity of 98% by HPLC is standard. Higher purity (99.5%) is recommended for premium formulations to avoid impurities that may affect product stability or cause skin sensitivity. Always verify purity via the provided COA.

Q2: How should ARA-290 peptide be stored after reconstitution?
Reconstituted ARA-290 peptide in sterile water or PBS should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles. Use within 3 months for maximum stability. For short-term use (1–2 days), store at 4°C in a sealed vial.

Q3: Can ARA-290 peptide be used in combination with other peptides in a single formulation?
Yes, ARA-290 is compatible with most water-soluble peptides. However, avoid mixing with high-concentration metal ions or strong reducing agents. Conduct a small-scale compatibility test at 0.1% concentration before full production. The peptide remains stable in pH 5.5–7.5 buffers.

Core Molecular Specs & Technical Index

ARA 290 peptide is a synthetic, 11-amino acid peptide derived from the erythropoietin (EPO) helix B domain. It is designed for laboratory research and cosmetic raw material applications, targeting specific receptor pathways without erythropoietic activity. This peptide is primarily sourced by B2B buyers including cosmetic formulation labs, peptide research facilities, and bulk raw material distributors. The core value of ARA 290 lies in its high specificity and stability, making it a preferred choice for advanced formulation studies.

Basic Properties and Purity Standards

ARA 290 peptide is supplied as a lyophilized powder with a molecular weight of approximately 1,200 Da. The standard purity for research-grade material is ≥98% as determined by HPLC analysis. For cosmetic raw material applications, purity levels of ≥99% are recommended to ensure batch-to-batch consistency and minimal impurity interference. High-purity ARA 290 peptide exhibits a white to off-white appearance and is soluble in water or DMSO at concentrations up to 10 mg/mL.

Technical Index Checklist

• Purity: ≥98% (HPLC) for research; ≥99% for cosmetic formulations
• Molecular Weight: 1,200 Da ± 50 Da
• Solubility: ≥10 mg/mL in water or DMSO
• Storage: -20°C for long-term; 2-8°C for short-term (up to 4 weeks)
• Stability: Lyophilized form stable for 24 months under recommended conditions

Industry data indicates that over 85% of peptide-related quality issues in cosmetic formulations stem from purity levels below 98%. Laboratories sourcing ARA 290 peptide with verified HPLC certificates report a 40% reduction in batch failure rates during formulation trials.

Manufacturing & Quality Control

The production of ARA 290 peptide involves solid-phase peptide synthesis (SPPS) using Fmoc chemistry. This method ensures high coupling efficiency and minimal side reactions. After synthesis, the crude peptide undergoes cleavage and deprotection, followed by purification via preparative HPLC. The final product is lyophilized under controlled conditions to preserve structural integrity.

Purification and Testing Protocols

Quality control for ARA 290 peptide includes multiple analytical steps. Each batch is tested for purity using analytical HPLC, mass spectrometry (MS) for molecular weight confirmation, and amino acid analysis to verify sequence accuracy. Third-party testing is recommended for cosmetic raw material applications, including endotoxin testing (≤1 EU/mg) and microbial limit tests. Certificates of Analysis (CoA) should accompany each shipment, detailing purity, retention time, and storage conditions.

Certification Requirements

• HPLC purity report with ≥98% or ≥99% specification
• Mass spectrometry (MS) confirmation of molecular weight
• Amino acid analysis for sequence verification
• Endotoxin testing (≤1 EU/mg) for cosmetic-grade material
• Microbial limit test (total aerobic count ≤100 CFU/g)

Commercial Application Scenarios

ARA 290 peptide is utilized across multiple B2B sectors. In cosmetic formulation, it is incorporated into serums and creams targeting skin barrier support and anti-aging properties. Laboratories use it for receptor binding studies and cellular signaling research. Bulk wholesale buyers source ARA 290 for large-scale formulation development and distribution to downstream clients.

Cosmetic Formulation Use

In cosmetic applications, ARA 290 peptide is typically added at concentrations of 0.1-1.0% in water-based formulations. It is compatible with common cosmetic ingredients such as hyaluronic acid, glycerin, and botanical extracts. Stability testing shows that formulations containing ARA 290 maintain peptide integrity for up to 12 months when stored at 2-8°C. Formulators should avoid high pH conditions (above 7.5) to prevent degradation.

Laboratory Research Applications

Research laboratories use ARA 290 peptide for in vitro studies involving cell culture models. Typical concentrations range from 10 nM to 1 µM depending on the assay. The peptide is often reconstituted in sterile water or PBS and used within 24 hours to maintain activity. Bulk research buyers often request custom packaging sizes, such as 10 mg or 50 mg vials, to minimize freeze-thaw cycles.

Bulk Wholesale Distribution

Wholesale buyers of ARA 290 peptide require consistent supply chains and batch-to-batch reproducibility. Large orders (100 mg to 1 kg) are typically shipped in sealed, light-protected containers with temperature monitoring. Volume discounts are available for orders exceeding 500 mg, with lead times of 2-4 weeks for custom synthesis.

ara 290 peptide VS Ordinary Low-Grade Peptides

Bulk Purchase Selection Guide

When sourcing ARA 290 peptide for laboratory or cosmetic use, buyers must avoid common pitfalls. The most frequent issues include receiving material with purity below 98%, incorrect molecular weight due to incomplete synthesis, and lack of proper documentation. Selection standards should include verification of HPLC chromatograms, MS spectra, and third-party testing reports.

Common Pitfalls in Sourcing

• Accepting material without a Certificate of Analysis (CoA)
• Ignoring endotoxin levels for cosmetic applications
• Purchasing from suppliers without ISO 9001 certification
• Failing to request batch-specific stability data

Buyer Checklist

• Request HPLC purity report with retention time and area percentage
• Verify molecular weight via MS or MALDI-TOF
• Confirm storage and shipping conditions (cold chain if needed)
• Ask for endotoxin and microbial test results
• Check supplier references and industry reputation

Core Product Advantages

ARA 290 peptide offers distinct advantages for B2B buyers. Purity is guaranteed at ≥98% for research and ≥99% for cosmetic applications, ensuring minimal batch variation. Stability under recommended storage conditions allows for long-term inventory management without degradation. Cost performance is optimized through efficient synthesis and purification processes, providing competitive pricing for bulk orders. Technical support includes access to formulation guidelines, stability data, and custom packaging options.

Frequently Asked Questions

Q1: What is the recommended purity level for ARA 290 peptide in cosmetic formulations?
For cosmetic raw material applications, a purity level of ≥99% is recommended. This ensures minimal impurities that could affect formulation stability or cause adverse reactions. Always request a Certificate of Analysis (CoA) with HPLC data to verify purity before use.

Q2: How should ARA 290 peptide be stored for long-term laboratory use?
Store lyophilized ARA 290 peptide at -20°C in a light-protected, airtight container. Under these conditions, the peptide remains stable for up to 24 months. For short-term use (up to 4 weeks), storage at 2-8°C is acceptable. Avoid repeated freeze-thaw cycles by aliquoting into single-use vials.

Q3: What documentation should I request when purchasing ARA 290 peptide in bulk?
Request a Certificate of Analysis (CoA) including HPLC purity, mass spectrometry confirmation, amino acid analysis, endotoxin levels, and microbial limit tests. For cosmetic-grade material, also ask for stability data and a material safety data sheet (MSDS). Ensure the supplier provides batch-specific documentation for traceability.